PCR Woes
(To the tune of "She'll Be Comin' Round the Mountain")
Copyright 2008 Tony Rianprakaisang

I am very pleased that Mr. Tim Karplus has made a professional recording of this song (complete song downloadable HERE) and others can be downloaded HERE. You will need to unzip the file to release the MP3. I am providing all songs for free download, but hope you will support this effort by purchasing my Metabolic Melody albums or calendar at the URLs at the bottom of the lyrics to this song.

First you must design the primers- PCR!
Make sure that they won't form dimers - PCR!
Then you check the melting T's
A's and T's are two degrees
And it's four for G's and C's in PCR!

Oh the thermocycler's set for PCR!
If this fails you'll be upset with PCR!
First you melt and did I mention
It's anneal and then extension
Copying is the intention - PCR!

Many times the protocol you've tried to fix
Checking all your tubes and then your mastermix
Looking for contamination
As you build up your frustration
If there's no amplification - PCR!

You can use reverse transcription - PCR!
Surely you'll throw a conniption - PCR!
Other types may give you trouble
Oh there's inverse and there's bubble
Your anxiety will double - PCR

Oh you've really got to amplify this strand
Hope that when you run your gel you've got a band
Didn't get what you expected
Damn this project you selected
Now you're feeling quite dejected - PCR!

Complete list of Metabolic Melodies HERE

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